Journal: Bioactive Materials

Article Title: Precisely regulated physically-crosslinked carriers enable synergetic release of bioactive factors for MSC-mediated cartilage regeneration

doi: 10.1016/j.bioactmat.2026.01.009

Figure Lengend Snippet: In vivo chondrogenesis of hydrogels incorporated with different combinations of PSF and KSF. (a) Alcian blue and Safranin O staining of different combinations after 21-day implantation. G1: 5 % β-sheet PSF +5 % β-sheet KSF; G2: 15 % β-sheet PSF +30 % β-sheet KSF; G3: 30 % β-sheet PSF +40 % β-sheet KSF; G4: 40 % β-sheet PSF +50 % β-sheet KSF. Scale bar = 200 μm. (b–d) Quantitative analysis of IL-1β, IL-6 and TNF-α surrounding defect cartilage 1 week and 3 weeks after operation. (e, f) Macroscopic and MRI observations of rat femoral condyles at week 6 and 12. Red circles and red arrows show the original defect zone under macroscope and MRI respectively. Scale bar = 1 mm. (g) Relative ratio of average optical density (versus G1) that shows the staining intensity of Alcian blue and Safranin O staining. (h) ICRS scoring of macroscopic evaluations. (i) The repaired cartilage using a nanoindentation instrument. Scale bar = 1 cm. (j, k) Reduced modulus and hardness of the regenerated cartilage. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.

Article Snippet: The suspension was centrifuged at 10,000 rpm for 10 min at 4 °C, and the supernatant was collected, and Elisa assay was performed following the manufacturer's instructions for rat IL-1β, IL-6, and TNF-α ELISA kits (Elabscience, China).

Techniques: In Vivo, Staining

Journal: Renal Failure

Article Title: Roxadustat attenuates contrast-induced acute kidney injury potentially mediated via the Nrf2/HO-1 signaling pathway

doi: 10.1080/0886022X.2026.2672811

Figure Lengend Snippet: The effect of ROX on inflammation and oxidative stress after CI-AKI. (A) Serum concentrations of TNF-α, IL-6 and IL-1β were measured by ELISA; (B) The mRNA levels of TNF-α, IL-6 and IL-1β in the kidney were assessed though RT-qPCR. (C) The concentrations of MDA in Kidney tissue was measured by ELISA; (D) The concentrations of GSH in Kidney tissue was measured by ELISA; (E) The concentrations of SOD in Kidney tissue was measured by ELISA; The data were represented as the mean ± S.D ( N = 10). *** p < 0.001 (CIN vs. Ctrl); # p < 0.05; (ROX vs. CIN); ^ p < 0.05 (ML vs. ROX).

Article Snippet: ELISA Kits for blood creatinine (Cr), urea nitrogen (BUN), interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 were acquired from Bioengineering Institute of Nanjing Jiancheng (Nanjing, China, C011–2, C013-1, A0101, A0119, A0107).

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR